Laboratory Guide to Gnotobiotic Research

Laboratory Guide to Gnotobiotic Research

Fletcher, Craig A.; Sartor, Ryan Balfour; Bower, Maureen A.; Rogala, Allison R.

Taylor & Francis Ltd

10/2028

320

Mole

Inglês

9781138305892

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Introduction. History of Gnotobiotics. The Germ Free Animal. Getting Started. Establishing a Gnotobiotic Facility. Architectural Design of the Facility. Stocking Items for the Facility. The Sterilization Process. Setting up the Isolators. The Addition of Mice into the Facility. Maintenance and Experimental Manipulation. Continuous Testing of Sterility. Breeding Management. Experimental Uses of Gnotobiotic Mice. Use of Isopositive Caging. Shipping Mice. Isolator Disassembly & Repair. Management & Personnel. Billing and Tracking of Orders. The Future of Gnotobiotic Research. Glossary of Terms.
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Germ free laboratory research;Microbiota in health and disease;Managing animal resources;Rederivation into GF isolators;Humane care and use of laboratory animals;Laboratory animal bacteriology